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Pass-through autoclaves Systec H-Series 2D

Equipped with the same state-of-the-art technology. The 2D series has the same modern design but now with two doors for fitting between separating walls.

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Systec GmbH

Konrad-Adenauer-Straße 15
35440 Linden
Germany

Tel. +49 (0) 6403 67070-0
Fax +49 (0) 6403 67070-222

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Sterilization of liquids

Heating up

The actual sterilization time of e.g. 15 to 20 minutes at 121 °C is only a fraction of the total time involved for an autoclave procedure. Especially in the case of sterilizing liquids, the heating up and cooling down phases are considerably longer.

The conventional procedure

In previously used conventional systems, even if the intended sterilization temperature has been reached within the autoclave, the liquids to be sterilized are often only at about 100 °C; the temperature equilibrium time between chamber and liquids normally takes much longer.

Up to 50 % shorter heat-up times as standard

Due to the combined temperature and pressure regulation, the chamber pressure is increased during the heat-up phase. The result: more rapid temperature equilibrium in the liquids and a shorter heat-up time.

Heating up
Heating up

Cooling

The cooling process for liquids is also very slow; this is because, without active rapid cooling, the heat can only be reduced to below 100°C by dissipating the heat via the chamber insulation by radiation (see diagram: conventional cooling).

New system- and process technology now make it possible to substantially reduce the overall time required for the sterilization process. This means that several hours of time can be saved! It also means that the media is not exposed to heat unnecessarily long time (see diagram: rapid cooling).

Systec offers many functions for its autoclaves guaranteeing safe liquid sterilization processes at higher productivity. Many of these functions are standard or available as options depending on the model range selected.

Standard functions in all models
  • Temperature- and pressure-dependent door locking in line with international standards and regulations.
  • Redundant process control; temperature and pressure are continuously monitored and controlled during the entire sterilization cycle.
  • Rapid heat-up via optimized heat transfer to the liquid media.
  • Flexible PT-100 temperature sensor for temperature measurement in a reference vessel:
    - Guarantees attainment of the desired sterilization temperature in the liquid media.
    - Guarantees cooling of the liquid media to a temperature that is safe for removal.


Systec supplies autoclaves guaranteeing precise sterilization processes, safe handling and increased productivity. Numerous cooling functions are available for liquid sterilization.

Various optional rapid cooling systems enable the cooling times for liquids to be significantly reduced. This conserves culture media and makes for efficient utilization of the autoclave.

In addition to conventional cooling by regulated steam exhaust down to 100 °C and subsequent very slow self-cooling down to 80 °C, optional cooling systems for rapid cooling are available.

  • Cooling with ambient air ventilation.
  • Mantle cooling with cooling water.
  • Mantle cooling with cooling water and support pressure.
  • Radial ventilator for air circulation and accelerated heat removal from the chamber.
  • Ultracooler.
  • Spray cooling with recirculated and recooled sterile water and support pressure.

Conventional cooling/Rapid cooling
Conventional cooling/Rapid cooling

Water cooling with support pressure

Permanently under control

During the entire sterilization process, a flexible PT-100 temperature sensor monitors the water temperature in a reference vessel. It is thus guaranteed that the sterilization period begins only once the sterilization temperature has been attained in the liquid to be sterilized.

The cooling temperature is also constantly monitored. In accordance with relevant standards, to prevent delayed boiling, the lid can only be opened once the temperature of the liquid has been reduced to at least 80 °C.

The use of support pressure in the form of sterile-filtered compressed air during the cooling phase reliably prevents the culture medium from boiling.

Advantages:

  • No loss of liquid due to boiling of the culture media.
  • Improved productivity from reduced cycle times and the full utilization of the filling volume in each bottle.
  • Prevention of delayed and over-boiling.
  • Prevention of the risk of bottles bursting during or after sterilization.
  • Prevention of re-contamination by the use of hermetically sealed bottles during sterilization.
  • Reduction of cooling time by up to 60 %.

Water cooling with support pressure
Water cooling with support pressure

Radial Ventilator

Together with water cooling with support pressure, the ventilator ensures accelerated removal of heat from the sterilization items to the cooled chamber mantle. The ventilator is located in the lid of the chamber (no reduction of chamber depth!) and is driven by a magnetic motor fitted outside under the cover.

  • Ventilation performance 250 m³/h.
  • Reduction of cooling time by up to 70 %.

Radial Ventilator
Radial Ventilator

Ultracooler

In conjunction with optional water cooling system with support pressure and Radial Ventilator Systec succeeded to reduce again the cooling time and hence the overall sterilization time considerably through integration of an additional heat exchanger.

  • Reduction of cooling time by up to 90 %.
  • Depending on the load, cooling times between 15 and 60 minutes can be achieved.

Ultracooler
Ultracooler

Fast heat-up at standard as well as options for fast recooling

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Sterilization of solids and waste in disposal bags

Vacuum system

Typical solids are e.g. pipette tips (in boxes), empty glassware and waste in bags as wall as porous materials such as filters or fabrics. For this type of sterilization, it is important to remove all air from the products to be sterilized to ensure precise, reproducible and validatable sterilization.

The vacuum device removes the air highly effectively from solids, tubing, porous materials, fabrics and disposal bags; in this way, the steam is able to penetrate completely. The process includes a fractionated pre-vacuum phase in combination with the standard steam generator. Only in this way it is possible to achieve validatable sterilization of porous materials, solids, fabrics or waste in bags.

Vacuum system
Vacuum system

Superdry – for drying solids

This optional accessory increases the drying efficiency for solids and porous materials such as filters and fabrics. Heat energy from the standard steam generator is transferred to the heating coils around the body of the sterilization chamber and is used for drying. Deep-vacuum drying using the optional vacuum device in conjuction with Superdry avoids the necessity for subsequent drying in a separate drying cabinet.

Superdry
Superdry

Vacuum system for efficient sterilization and drying

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Sterilization of hazardous biological substances

Permanently monitored – exhaust air filtration with condensate inactivation

For the sterilization of hazardous biological substances, Systec autoclaves can be fitted with an optional air exhaust filtration system.

The autoclavable sterile filter comprising a filter cartridge with PTFE membrane, pore size 0.2 μm, incorporated in a pressureresistant housing, easy replaceable. The filter is automatically sterilized during each sterilization process, monitored by a PT-100 temperature sensor.

The condensate is retained inside the pressure vessel during the heating and sterilization phases and thus also sterilized. Through air exhaust filtration and condensate inactivation, it is ensured that no microorganisms can escape before end of the sterilization phase.

This ensures that all gases and liquids representing a hazard if they were to be released into the atmosphere are filtered or sterilized in-line.

Exhaust air filtration
Exhaust air filtration

Highest safety through air exhaust filter

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Sterilization goods and sterilization procedures

Select the right process for every sterilization application

As already described, several options are available that are necessary to obtain correct and validatable results and rapid cooling times, especially in the case of liquids. The options available depend on the items to be sterilized. It is thus important for you to think carefully about your requirements so that the autoclave can be optimally configured to the tasks on hand. A validatable sterilization process of confirmable biological efficiency can only be obtained if the correct instrument configuration is used. The table below provides help in establishing the desired configuration; however, we recommend obtaining additional advice from our experts.

  • Procedure: Ventilation Cooling Drying Other
    Gravitation Simple
    pre-vacuum
    Pulsed excess
    pressure
    Fractionated
    pre-vacuum
    Conventional cooling with slow pressure release Rapid cooling system with support pressure Surface drying without vacuum Drying with subsequent vacuum + Superdry Exhaust air filtration
  • Liquids + ? - - ? + - -
  • Unpacked non hollow items + + + + ? +
  • Porous materials
    (filters, fabrics)
    - ? ? + - +
  • Hollow items (pipette tips, empty glassware, tubes and hoses) - - - + - +
  • Contaminated waste in destruction bags - - ? + - - +
+ Recommended procedure   ? Possibly acceptable  - Not possible


The right procedure for efficient sterilization

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